GAS CHROMATOGRAPHY AND MASS SPECTRUM ANALYSIS OF CATHARANTHUS PUSILLUS MURRAY G. DON (APOCYANACEAE)

Abstract

In the present study the bioactive compounds of Catharanthus pusillus, aerial parts and root extract were used to
evaluate by Gas chromatography and mass spectrum (GC-MS) method. 50g of fine powder was packed with No.1 Whatman filter
paper and placed in soxhlet apparatus along with methanol. The crude extract were collected and dried at room temperature, 30˚C
after which yield was weighed and then performed to GC-MS analysis. Nine components from the aerial part and root of the
above said plant were identified. The major compounds are identified in aerial parts such as Pregn-16-en-20-one, 11,18-bis
(acetyloxy)-3,9-epoxy-3-methoxy-, (3à,5á,11à)-(15.60%),9-Octadecenoic acid (14.03%), 3-Methyl-trans-2,3-epoxycyclohexan-1-
ol(8.53%), 1H-Purin-6-amine, [(2-fluorophenyl)methyl], (7.47%), Octadecanoic acid (6%). In roots, the major compounds are
stigmasterol (13.26%), ç-Sitosterol (11.39%), 2, 5-Dimethoxy-4-ethylamphetamine (4.68%). This study helps to explore the
potential compounds responsible for the biological activities of antimicrobial, antioxidant, antidiabetic and anticancer for
application of drug formation in pharmaceutical fields.