Utilization of RP-HPLC fingerprinting analysis for the identification of diterpene glycosides from Stevia rebaudiana

Abstract

Identification of diterpene glycosides present in the commercial extracts of the leaves of Stevia rebaudiana was achieved by employing a reversed-phase (RP)-high performance liquid chromatography (HPLC) method using charged aerosol detector (CAD). Using the RP-HPLC method, the individual retention times for 26 naturally occur- ring ent-kaurane diterpene glycosides identified from S. rebaudiana were identified. Further, the HPLC method  has been successfully utilized in identifying the presence of various steviol glycosides from the crude stevia ex- tracts obtained from Pure Circle, Stevia Food (ShenZhen) Company Limited, Heilongjinag Hailin Farm Stevia Refi- nery, Qingdao Sanfood Company Limited, DIC LifeTec Company Limited, and Daepyung Company Limited.